三疣梭子蟹几丁质酶基因(PtCht6)的克隆及其在免疫中的功能分析     

宋柳  吕建建  王磊  孙东方  刘萍 《海洋与湖沼》2019,50(5):1080-1090

几丁质酶(chitinase)在甲壳动物的蜕皮、消化和免疫等很多生物学过程中发挥重要功能,为深入探讨几丁质酶的免疫防御机制,本实验利用RACE技术克隆了三疣梭子蟹(Portunus trituberculatus)PtCht6基因。该基因cDNA全长2736bp,开放阅读框(ORF)2103bp,编码700个氨基酸,具有几丁质酶GH18家族典型特征。利用实时荧光定量技术分析PtCht6在三疣梭子蟹不同组织、不同蜕皮阶段、不同病原感染以及低盐胁迫(11)下的表达特征,结果显示:PtCht6在各组织中均有表达且在肝胰腺中的表达量最高;在不同蜕皮时期的肝胰腺中,其表达量由蜕皮后期(A/B)、蜕皮间期(C)、蜕皮前期(D)依次递减(P0.05);人工感染WSSV和副溶血弧菌后.PtCht6的表达量在肝胰腺中分别于12h、72h达到最大值,在血细胞中均于12h达到最大值,且相较于对照组,整体显著上调表达(P0.05);低盐胁迫能够显著抑制该基因的表达,最高抑制73倍(P0.05)。同时发现,低盐环境下感染WSSV后,该基因达到峰值的时间明显延迟(至少延迟12h.P0.05)。该研究结果预示PtCht6作为免疫因子参与三疣梭子蟹的病原防御,且低盐胁迫在一定程度上抑制了该基因的免疫功能。  相似文献   

Closed-loop randomized kinodynamic path planning for an autonomous underwater vehicle     

《Applied Ocean Research》2019

A randomized kinodynamic path planning algorithm based on the incremental sampling-based method is proposed here as the state-of-the-art in this field applicable in an autonomous underwater vehicle. Designing a feasible path for this vehicle from an initial position and velocity to a target position and velocity in three-dimensional spaces by considering the kinematic constraints such as obstacles avoidance and dynamic constraints such as hard bounds and non-holonomic characteristic of AUV are the main motivation of this research. For this purpose, a closed-loop rapidly-exploring random tree (CL-RRT) algorithm is presented. This CL-RRT consists of three tightly coupled components: a RRT algorithm, three fuzzy proportional-derivative controllers for heading and diving control and a six degree-of-freedom nonlinear AUV model. The branches of CL-RRT are expanded in the configuration space by considering the kinodynamic constraints of AUV. The feasibility of each branch and random offspring vertex in the CL-RRT is checked against the mentioned constraints of AUV. Next, if the planned branch is feasible by the AUV, then the control signals and related vertex are recorded through the path planner to design the final path. This proposed algorithm is implemented on a single board computer (SBC) through the xPC Target and then four test-cases are designed in 3D space. The results of the processor-in-the-loop tests are compared by the conventional RRT and indicate that the proposed CL-RRT not only in a rapid manner plans an initial path, but also the planned path is feasible by the AUV.  相似文献   

浙江省三疣梭子蟹产量波动及潜在影响因素分析     

胡荧斌  徐美佳  王天姿  何毓钦  王迎宾 《海洋科学》2019,43(2):69-73

依据2000—2015年浙江省三疣梭子蟹(Portunustrituberculatus)渔获量的数据,以及影响渔获量的因素(管理类因素、环境类因素和营养关系类因素等共11个因子),使用广义加性模型(GAM)对各类因素对三疣梭子蟹渔获量的影响进行了分析。结果显示,赤潮面积、捕捞努力量和放流数量对三疣梭子蟹渔获量影响显著(P0.01)。3个因子的累积解释比率达98.3%,其中,赤潮的解释比率最大,达到47.2%,捕捞努力量的解释比率为37.8%,放流数量的解释比率为13.3%。三疣梭子蟹渔获量随着赤潮面积增加有所减少,随着捕捞努力量的加大有迅速增加的趋势,与放流尾数呈现正相关趋势。三疣梭子蟹渔获量的波动受诸多因素的影响。今后需开展更深入的研究,找到可能影响三疣梭子蟹渔获量的其他关键因素。  相似文献   

非连续变形分析中块体大转动问题研究     

巩师林  凌道盛  胡成宝  钮家军 《岩土力学》2020,41(11):3810-3822

原有非连续变形分析（DDA）采用一阶近似后的位移增量表达式更新块体构形，推导相关子矩阵，且对不同时步计算出的应变增量直接叠加，当模拟的块体发生大转动时往往会产生较大误差。为考虑块体转动与变形的耦合作用，引入先变形、后转动的块体位移增量表达式。重新推导了惯性力子矩阵，将块体转动时的离心力与科氏力加到荷载矩阵中。计算时对应变分量及其相关变量进行坐标变换与修正，并采用新引入的位移增量表达式计算块体顶点位移，进行后接触修正与更新块体构形。数值算例表明，改进后的程序能够消除转动带来的误差，自动考虑了块体转动时离心力和科氏力引起的变形，应变计算精度更高。改进方法克服了块体体积自由膨胀、应变场畸变等问题，给出了合理的块体应变。  相似文献   

Turbulence Diffusion and Energy Spectrum Analysis of Large-scale Current Sheets in Flares     

《Chinese Astronomy and Astrophysics》2021,45(4):470-493

Magnetic reconnection (MR) is one of the most important physical processes for many dynamical phenomena in the universe. Magnetohydrodynamical (MHD) simulation is an effective way to study the MR process and the physical pictures related to the MR. With different parameter setups, we investigate the influences of the Magnetic Reynolds number and spatial resolution on the reconnection rate, numerical dissipation, and energy spectrum distribution in the MHD simulation. We have found that the magnetic Reynolds number $R_m$ has definite impact on the reconnection rate and energy spectrum distribution. The characteristic time for entering into the non-linear phase will be earlier as the Reynolds number increases. When it comes to the tearing phase, the reconnection rate will increase rapidly. On the other hand, the magnetic Reynolds number affects significantly the Kolmogorov microscopic scale $l_{\mathit{ko}}$, which becomes smaller as $R_m$ increases. An extra dissipation is defined as the combined effect of the numerical diffusion and turbulence dissipation. It is shown that the extra dissipation is dominated by the numerical diffusion before the tearing mode instability takes place. After the instability develops, the extra dissipation rises vastly, which indicates that turbulence caused by the instability can enhance the diffusion obviously. Furthermore, the energy spectrum analysis indicates that $l_{\mathit{ko}}$ of the large-scale current sheet may appear at a macroscopic MHD scale very possibly.  相似文献   

连续台风时期香港区域的水汽变化分析     

周苏娅  闻德保  梅登奎 《大地测量与地球动力学》2020,40(1):82-86

利用香港卫星定位参考站网GNSS观测数据,提取强热带风暴"塔拉斯"与热带风暴"洛克"影响期间各测站天顶方向对流层延迟,反演香港区域大气可降水量;根据香港区域49个天文台气象站提供的实测降雨量数据,分析大气可降水量与实际降雨量的相关性,以及两次台风对香港区域水汽时空分布的不同影响。结果表明,大气可降水量在台风影响前期均上升,在大量降雨后回落,但在连续台风的间歇期间,仍高于台风来临前的水平;水汽累积是大量降雨的前提条件,当水汽累积量相近时,水汽累积时长与累积降雨量呈正相关;台风期间大气可降水量值超过65 mm的区域面积与台风等级相关,台风路径对局部水汽分布有一定的影响。  相似文献   

Intercomparison of measurements of bulk snow density and water equivalent of snow cover with snow core samplers: Instrumental bias and variability induced by observers     

J. Ignacio López-Moreno  Leena Leppänen  Bartłomiej Luks  Ladislav Holko  Ghislain Picard  Alba Sanmiguel-Vallelado  Esteban Alonso-González  David C. Finger  Ali N. Arslan  Katalin Gillemot  Aynur Sensoy  Arda Sorman  M. Cansaran Ertaş  Steven R. Fassnacht  Charles Fierz  Christoph Marty 《水文研究》2020,34(14):3120-3133

Manually collected snow data are often considered as ground truth for many applications such as climatological or hydrological studies. However, there are many sources of uncertainty that are not quantified in detail. For the determination of water equivalent of snow cover (SWE), different snow core samplers and scales are used, but they are all based on the same measurement principle. We conducted two field campaigns with 9 samplers commonly used in observational measurements and research in Europe and northern America to better quantify uncertainties when measuring depth, density and SWE with core samplers. During the first campaign, as a first approach to distinguish snow variability measured at the plot and at the point scale, repeated measurements were taken along two 20 m long snow pits. The results revealed a much higher variability of SWE at the plot scale (resulting from both natural variability and instrumental bias) compared to repeated measurements at the same spot (resulting mostly from error induced by observers or very small scale variability of snow depth). The exceptionally homogeneous snowpack found in the second campaign permitted to almost neglect the natural variability of the snowpack properties and focus on the separation between instrumental bias and error induced by observers. Reported uncertainties refer to a shallow, homogeneous tundra-taiga snowpack less than 1 m deep (loose, mostly recrystallised snow and no wind impact). Under such measurement conditions, the uncertainty in bulk snow density estimation is about 5% for an individual instrument and is close to 10% among different instruments. Results confirmed that instrumental bias exceeded both the natural variability and the error induced by observers, even in the case when observers were not familiar with a given snow core sampler.  相似文献   

基于MATLAB的铁路路基沉降预测优化方法     

刘鹏鹏  李仲勤 《北京测绘》2020,(4):580-583

高速铁路路基的工后沉降严重影响着行车安全。在已有的预测模型中,所采用的初始数据往往不能满足等时间周期采集,而且还会伴随着一系列不可避免的观测误差,模型本身的误差累计,不能进行长期预测。文中利用最小二乘原理对初始值进行拟合改进,采用Lagrange插值方法将非等间隔序列转为等间隔序列,并基于新陈更替GM(1,1)模型利用MATLAB建立沉降预测模型;在此基础上,提出对模型残差进行GM改正以提高模型精度的方法。研究表明,通过对初始值序列改正后的模型具有较好的适应性,优化改进后的模型预测误差小,预测精度优于新陈更替GM(1,1)模型。  相似文献   

复杂地形景区三维导览图的设计与实现     

吴思锴  李百寿  郭家豪  李雪  张卫平 《北京测绘》2020,(1):38-43

为满足复杂地形景区对三维地图导览的需求,构建更为清晰的三维地图模型,提供良好的三维导览地图设计方案显得尤为重要。为了更好地与卫星影像进行贴合,DEM数据的采样密度要与卫星影像的分辨率一致。本文通过对比当下常用的四种空间插值方法的适用范围与运用特点,设置相关阈值及权重,直观比较了四种插值方法产生的插值结果,选择更适合复杂景区DEM插值的插值方法并对其进行精度提升,以此得到符合精度要求的DEM数据。最后利用相应地区的卫星影像进行地图投影及影像贴图,两种数据结合构建复杂景区的三维地图模型,给游客带来更为精确直观的定位信息和空间要素信息。  相似文献   

基于SPE与SPATT的水体中麻痹性贝类毒素检测方法构建与应用   总被引：1，自引：0，他引：1  

张亚亚  闫国旺  吴海燕  谭志军  张志华  江涛  李玉 《海洋与湖沼》2020,51(2):298-306

为实现麻痹性贝类毒素(paralytic shellfish poisoning,PSP)的实时监控与提前预警,本研究构建了基于固相萃取技术(solid phase extraction,SPE)与固相吸附毒素跟踪技术(solid phase adsorption toxin tracking,SPATT)的水体中PSP检测方法,重点优化了吸附材料及前处理方法,评价了回收率、检出限等指标,并将方法应用于2019年春季秦皇岛山海关海域PSP消长过程的监测中,比较评估了两种方法的监控预警效果。结果表明:SPE方法选用ENVI-Carb 500mg/6mL固相萃取柱,过样体积为50mL,13种PSP组分的平均回收率为82.2%±10.0%、检出限为4.0-20.0ng/L;SPATT方法选用SP207大孔吸附树脂,洗脱时间为静置Id最佳,整体回收率约为9.2%;在实际应用中,结合产毒藻密度及贻贝富集毒素含量的变化,发现SPE方法的检测结果可实时表征海域PSP风险状况,对于贻贝中PSP的预警效果也显著优于SAPTT方法,后者不仅因监控方式相对滞后一个监测周期,且灵敏度及准确性均较差。对于秦皇岛海域,当SPE方法检测结果达到100ng STX eq/L时,该海域贻贝中PSP残留将具有潜在的食用安全风险,跟踪过程表明这一阈值可提前两周预警贻贝富集毒素含量超出我国限量标准(800μg STX eq/kg),这对于强化风险警示并制定防范措施具有积极作用。  相似文献